Pregermination treatments


Suggested pregermination treatments for key nitrogen fixing tree and shrub species that tolerate acid soils.


Species

Treatment

Seeds/kg

Acacia aneura

B 1 min, S

75,000–80,000

Acacia angustissima

B, S

90,000–100,000

Acacia auriculiformis

B 30 sec, S

30,000–90,000

Acacia holosericea

B 1 min, S

70,000–80,000

Acacia koa

B 2 min, S

8,500–20,000

Acacia mangium

B 30 sec, S

80,000–100,000

Acacia mearnsii

B 5 mins

48,000–85,000

Acacia melanoxylon

B, S

60,000–100,000

Albizia lebbeck

B 3 sec

6,000–16,000

Albizia saman

B 2 min, C, S

6,000–8,000

Alnus acuminata

R

200,000–2,000,000

Alnus glutinosa

R

Calliandra calothrysus

B

14,000–19,000

Casuarina cunninghamiana

R

1,800,000

Casuarina junghuhniana

R

1,000,000–1,600,000

Casuarina sumatrana

R

Chamaecytisus palmensis

B 1 min

Cedrelinga catenaeformis

B

Dalbergia nigra

C

10,000–30,000

Desmodium gyroides

R

500,000

Desmodium nicaraguense

R

500,000

Desmodium velutinum

R

500,000

Enterolobium cyclocarpum

C, S

800–2,000

Enterolobium contortisiliquum

C, B

Erythrina abyssinica

C

Erythrina berteroana

C

Erythrina poeppigiana

C

3,000–5,000

Erythrina fusca

C

Erythrina variegata

B 10 min

Flemingia macrophylla

R

50,000–80,000

Gliricidia sepium

C, B, or S

7,000–12,000

Inga acrocephala

R

Inga codonantha

R

Inga edulis

R

Inga marginata

R

Inga punctata

R

Inga spectibilis

R

Mimosa scabrella

B 3 mins

60,000–90,000

Mimosa caesalpinaefolia

B

Paraserianthes falcataria

B 10 mins

40,000–50,000

Pithecellobium dulce

R

Prosopis juliflora

B, S

Pterocarpus indicus

R

1,500–2,000

Robinia pseudoacacia

B 2 min, S

35,000–50,000

Stryphnodendron adstringens

B

Stryphnodendron excelsum

B


B: Pour boiling water over seeds (about five times as much water as the volume of the seeds), stir gently for 1 to 2 minutes (or as specified), and then soak in cold water for 12 to 24 hours.
C: Soak seeds in cold water for 12 to 24 hours.
S: Nick the round end of each seed with a file, knife, or nail clipper or rub the round end on a rough surface such as cement until the white interior is barely visible. Soak the treated seeds in cold water for 12 hours.
R: Recalcitrant seed; no treatment needed.


Source:
Briscoe, C.B., S.M. de Faria, V. Lambert, G. Minnick, and R. Russo. 1996. Growing Nitrogen Fixing Trees in Acid Soils. In: M.H. Powell (ed) Nitrogen Fixing Trees for Acid Soils - A Field Manual. Winrock International, Morrilton (AR), USA. p. 22.


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